Strengths, Challenges, Opportunities and Threats (SCOT) is a simple yet comprehensive way of assessing the positive and negative forces within and outside your organization so you can be better prepared to act effectively. It reminds the project leader to build on strengths, minimize challenges, seize opportunities, and counteract threats.

Assignment Prompt

Each student will perform a SCOT (formerly SWOT) analysis in their practice that identifies strengths, challenges, opportunities and threats to assist in making strategic plans and decisions in the implementation of the EBP.

Submit a 1-2 slide PowerPoint presentation of a SCOT analysis as it relates to your project.

Expectations

• easily found US sources and ( use nurse retention for critical care) 
• Length: 1-2 slides
• Format: Power Point with diagram
• Research: At least one peer reviewed reference within the last 5 years

BIOS242, Week 2-OL

Name:

Lab 2: Bacterial Isolation

Learning Objectives

· Explain the importance of bacterial growth for the investigation of pathological microorganisms

· Use aseptic techniques

· Explain the concept of a single colony

· Perform plate-streaking techniques

· Use selective media for isolation purposes

Techniques in Lab

· Colony screening

· Sterile technique

· Plate streaking

Introduction: Bacterial Isolation Virtual Simulation Lab
In the Bacterial Isolation simulation, you will investigate the cause of a contamination of poultry meat by a dangerous bacteria strain that is resistant to common antibiotics. After taking samples from the chicken farm, you will work in the virtual laboratory to isolate single colonies of the deadly bacteria among a variety of different species. To do so, you will learn how to work under sterile conditions, and you will be able to practice and perfect your plate streaking technique.

Identifying ampicillin resistant bacteria
The premise of the Bacterial Isolation lab is a report of ampicillin resistant bacteria in poultry meat. You will visit the place of origination, a chicken farm, in hopes of identifying the bacteria strain. The first task is to take a sample, which will contain a variety of bacterial strains, and from that, you must identify which strains are resistant to ampicillin by isolating single colonies.

Aseptic technique
In the Bacterial Isolation lab, you will learn how to use aseptic techniques—for example, remembering to turn on the Bunsen burner and sterile their loop in between streaks.

Plate streaking technique
In order to identify the specific bacteria strain, you will have to perform bacterial isolation using the plate streaking technique. There is an unlimited supply of agar plates, giving you the opportunity to practice this technique as many times as you like. Results are given immediately, as opposed to waiting a full 24 hours for incubation, as when performed in real life as opposed to in a virtual simulation. You will also streak a special Salmonella Shigella agar. The Salmonella Shigella agar contains a certain medium that only promotes growth of Gram-negative strains. Each of the different strains will exhibit a certain phenotype when grown on the Salmonella Shigella agar. By using this information you can identify the specific strain that is resistant to ampicillin. The sample will then be sent for further analysis to fully confirm the identity.

Will you be able to complete the task with your knowledge on bacterial isolation and successfully isolate the dangerous bacterial strain?

Questions:

1. Purpose: Please describe in complete sentences and in your own words, the purpose of this experiment.

2. Why is proper aseptic technique important in microbiology?

3. What is the importance of flaming the inoculating loop or needle before
and after each inoculation?

4. If you do not wait 10 – 20 seconds after flame sterilizing the inoculating instruments before obtaining the sample, what might be the consequences?

5. Why is it important to flame neck of the tubes immediately after uncapping and before recapping the tubes?

6. Reflection: Write 5 sentences on what you learned from this simulation, what did you like and what was something that you would not prefer to be in the simulation.

Grading Rubric:

4

Infection Topic

Top of Form

Bottom of Form

Module 06 Content

1.

Top of Form

Pick from MRSA, C-Diff, Tuberculosis, OR Varicella Zoster

Complete the 
ATI Systems Disorder template for your assigned infection topic. 
Every box on the template must be completed, a citation is needed for every box, and your reference list must be included (APA). 

Week 1 Discussion: Social Factors

I live in Lady Lake, FL. According to the U.S. Census Bureau in 2022, the city of Lady Lake’s population was 16,661, the median income was $42,088, and the percentage of people living in poverty was 14.0% (U.S. Census Bureau, 2022). The grocery store that I usually shop at and that is local is called Winn-Dixie. The Winn-Dixie I went to was located in Lady Lake FL. I did my research when I went grocery shopping yesterday and gathered the prices of the following, all were non-organic.

· 1 gallon of milk: $ 4.19 (whole milk)

· 1 loaf of bread: $4.19 (butter bread)

· 1 bag of apples (including price per pound): $5.99 (for a 3lb bag) /$2.39 per pound.

· 1 bag of carrots: $2.39 (baby-cut carrots)

· 1 large container of yogurt: $5.99 (Greek yogurt 32 ounces)

· 1 2-liter of soda: $1.49 (liter of coke)

· 1 bag of chips: $5.99 (bag of Doritos)

· 1 package of cookies: $4.19 (Chips Ahoy)

When it comes to buying groceries there are several social factors that could impact what groceries are purchased. Poverty levels can impact what groceries are purchased due to limited income. Those with limited income and are considered to be “in poverty” will rely more on cheaper and more affordable food options.  According to the US Department of Health and Human Services, “Low-income groups tend to rely on foods that are cheap and convenient to access but are often low in nutrients” (U.S. Department of Health and Human Services, n.d.).Cheaper food options may not be the most nutritious or healthy. Also, those who live in poverty might rely more on food that is processed and packaged, these foods will last longer helping them get the most out of their money.  We can look at what is gathered above, yogurt is $5.99 and will not last as long as cookies which is $4.19.

The second social factor that could impact what groceries are purchased would be availability. When grocery stores have a wide variety of fresh fruits and vegetables it can be easier for people to select those healthier options and incorporate them into their diets. If the grocery stores do not have these items readily available, then customers may be led to frozen processed fruits and vegetables. Availability and assortment are important for those who may not have the money or accessibility to other sources of fresh produce.

A community health nurse could use outreach as an intervention to address areas of food security. According to our book,
Community/Public Health Nursing, the definition of outreach is, “locates populations of interest or populations at risk and provides information about the nature of the concern, what can be done about it, and how services can be obtained” (Nies & McEwen, 2022). The community health nurse should focus on the poverty population recognizing that they are at risk for food security and reach out to them. Once information is gathered the CHN can then do further research on how we can help those in need and provide them the services they may need.

References

Nies, M., & McEwen, M. (2022).
Community/Public Health Nursing. Elsevier Health Sciences.

U.S. Census Bureau. (2022).
U.S. Census Bureau. Retrieved from https://www.census.gov/quickfacts/fact/table/ladylaketownflorida,US/PST045222?

U.S. Department of Health and Human Services. (n.d.).
U.S. Department of Health and Human Services. Retrieved from Access to Foods That Support Healthy Dietary Patterns: https://health.gov/healthypeople/priority-areas/social-determinants-health/literature-summaries/access-foods-support-healthy-dietary-patterns#:~:text=Low%2Dincome%20groups%20tend%20to,are%20often%20low%20in%20nutrients.&text=Fresh%20fruits%20and%20vegetab

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